microtubule associated protein map  (Cytoskeleton Inc)

Journal: Nutrition Research and Practice

Article Title: Modulatory effects of Hizikia fusiformis on the dopaminergic system in a neonatal habenular lesion-induced attention-deficit hyperactivity disorder-like rodent model

doi: 10.4162/nrp.2026.20.1.31

Figure Lengend Snippet: (A) The bar graph indicates the percentage of VEH in HF-induced MAP2 expression. (B, C) These bar graphs are similar to graph (A); however, they show the expression of NF (B) and SYP (C). Protein expression was analyzed using analysis of variance and Bonferroni post-hoc test. Statistical significance was set at P < 0.05. HF, Hizikia fusiformis ; MAP2, microtubule-associated protein 2; NF, neurofilament; SYP, synaptophysin; VEH, vehicle.

Article Snippet: The following were the primary antibodies used: mouse monoclonal anti-synaptophysin (SYP; catalog #: S5768; Sigma-Aldrich), rabbit polyclonal anti-tyrosine hydroxylase (TH; catalog #: E-AB-33093; Elabscience, Houston, TX, USA), mouse monoclonal neurofilament (NF; catalog #: sc-20012; Santa Cruz Biotechnology, Dallas, TX, USA), rabbit polyclonal anti-DAT (catalog #: PA1-4656; Invitrogen, Waltham, MA, USA), mouse monoclonal anti-DRD2 (catalog #: sc-5303; Santa Cruz Biotechnology), rabbit polyclonal anti-DRD1 (catalog #: E-AB-31152; Elabscience), mouse monoclonal anti-DRD3 (catalog #: sc-136170; Santa Cruz Biotechnology), rabbit polyclonal anti-DRD5 (catalog #: MBS2516950; MyBioSource, San Diego, CA, USA), mouse monoclonal anti-microtubule-associated protein 2 (MAP2; catalog #: sc-32791; Santa Cruz Biotechnology), and rabbit polyclonal anti-DRD4 (catalog #: E-AB-31153; Elabscience).

Techniques: Expressing

Journal: iScience

Article Title: Otic organoids: A model to study spiral ganglion neuron characteristics in Tmprss3-deficiency

doi: 10.1016/j.isci.2025.114355

Figure Lengend Snippet: Otic patterning and neuronal differentiation in WT and TMPRSS3-deficient organoids (A) Day 16 K2/8 WT organoid showing compartmentalization into ECAD + otocyst-like epithelium (red) and JAG1 + protruding domain (green). Schematic illustration (center) indicates spatial organization. (B, D) Day 25 K2/8 WT organoids expressing PAX8 and JAG1, together with SOX2 + progenitors and POU4F1 + neuronal precursors. (C, E) Day 25 K2/8 C4 KO organoids also expressed PAX8 and JAG1, but lacked SOX2 and POU4F1, indicating absence of progenitors and early neuronal specification. (F, G) Day 45 WT organoids display MAP2 + neurites co-expressing PRH (F) or POU4F1 (G). PRH is typically associated with type II SGNs, while POU4F1 marks type I SGNs, indicating the emergence of both major SGN subtypes at this stage. (H, H′) Day 70 WT organoids showing TMPRSS3 expression (red) co-localizing with PRH (green) in neuronal cell bodies. Higher magnification (H′) highlights TMPRSS3 + /PRH + neurons. Representative images were obtained from n ≥ 3 organoids per time point from four independent differentiation experiments. Scale bars, 100 μm (A–H) and 10 μm (H′). Nuclei were counterstained with DAPI (blue). See also .

Article Snippet: MAP2 , Aves Lab , MAP.

Techniques: Expressing

Journal: iScience

Article Title: Otic organoids: A model to study spiral ganglion neuron characteristics in Tmprss3-deficiency

doi: 10.1016/j.isci.2025.114355

Figure Lengend Snippet: Characterization of SGN-like neurons and glial cells in day-70 otic organoids (A) Day-70 organoids stained for VGLUT1 (green), MAP2 (white), and TUBB3 (red), confirming glutamatergic identity of neurons with neuritic extensions. VGLUT1 shows punctate labeling along MAP2 + neurites, consistent with its synaptic localization. (B) Dissociated day-70 organoid showing a representative SGN-like cell with bipolar morphology. The soma gives rise to two opposing neurites, co-expressing MAP2 (white), TUBB3 (red), and VGLUT1 (green). (C) S100β + glial cells (red), marking supporting glial populations within the organoids. (D) GFAP + glial cells (red), confirming astrocytic-like glial populations in the organoids. (E–E′) PRH + (green), TH + (red), and MAP2 + (white) cells, consistent with type II SGNs. (F–F′) CALB2 + (green) and MAP2 + (white), characteristic of type Ia SGNs. (G–G′) PROX1 + neurons (red) with MAP2 + (white) morphology, consistent with type Ib SGNs. PROX1 labeling appears predominantly perinuclear with partial overlap to DAPI, consistent with its function as a transcription factor. (H–H′) POU4F1 + (green), NF200 + (red), and MAP2 + (white) cells, characteristic of type Ic SGNs. Representative images were obtained from n ≥ 3 organoids per time point from four independent differentiation experiments. Scale bars, 100 μm (C–H), 50 μm (A), 20 μm (B), 10 μm (E′–H′). Nuclei were counterstained with DAPI (blue). See also .

Article Snippet: MAP2 , Aves Lab , MAP.

Techniques: Staining, Labeling, Expressing